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I spent this morning doing slot-blot filter binding assays, which are an experiment in which you measure protein-nucleic acid (DNA or RNA) interactions using a double membrane sandwich with a nitrocellulose membrane on top that traps proteins (and bound nucleic acids) and a nylon membrane below that binds any free nucleic acids. So I planned to make a video showing you how I set it up and run it - and I did - but I also found I had some stuff to say about experimenting in general so I put that stuff first and then the behind-the-scenes look at the blot-a-lot-a-lotting If you want way more details…. much more about slot blotting: http://bit.ly/bindingaffinity more about Kd: http://bit.ly/bindingaffinity more about phosphor storage screens: http://bit.ly/phosphorimaging blog form: https://bit.ly/slotblotalot

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Buffalo Bill's is a hotel and casino in Primm, Nevada, at the California state line. It is one of the Primm Valley Resorts, owned and operated by Affinity Gaming. It has 1,242 guest rooms and suites. The hotel is home to the Desperado roller coaster, a hypercoaster with a 225-foot drop and a top speed of 80 mph, as well as a pool in the shape of a buffalo. The hotel-casino has two hotel room towers: the Annie Oakley Tower (the A Tower) and the Buffalo Bill Tower (B Tower). It is named after Buffalo Bill. A tram links Buffalo Bill's to nearby Primm Valley Resort. The multi-car tram was designed by Schwager-Davis to their UniTrak standard. Despite its name, the tram actually runs on two guideways. Each car accommodates 24 people; the four-car trains can seat 96 people with no standees. The system can transport 2,200 people, per hour, per direction (pphpd). An attendant supervises each tram, although the tram is controlled by a computer. After disembarking at the Primm Valley Resort station, passengers can connect to a free single-car tram across the I-15 to Whiskey Pete's, although the connection takes a walk across the entire hotel. Please support my channel guys. Click like comment and subscribe for more videos. https://www.youtube.com/channel/UCjTBBaWcz5zNKVPf4nDYhTA/videos?disable_polymer=1

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