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To Purchase: https://bit.ly/3oD1WEa Find in Catalog: https://bit.ly/428g7iZ 80/20 Website: https://bit.ly/3peM7n3 Hello and welcome to 80/20’s product highlight series. Today we’re going to take a look at T-slot cover. T-slot cover is a great finishing touch to any project. It comes in a wide variety of colors, to allow your projects to really pop. It can also prevent anything from getting into the open T-slot. It’s available in both fractional and metric, and is compatible across a wide range of profile series. When it comes to installing the T-slot cover, that first thing you’ll want to do is decide which style is best for your project. We have our standard T-slot cover and we have economy T-slot cover that is a little bit lighter. Then we have a reduction T-slot cover. This style can also be flipped over and used to reduce the gab in the T-slot and hold your panels into place. And finally 80/20 now offers an aluminum T-slot cover, which is weather resistant, durable, and blends in great with the profile. No regardless of your choice, they all install the same way. So we’ve got our piece of T-slot cover cut to length, we’ll put it over the open T-slot and pop it right into place. Now if you ever want to replace it, or simply take it out, take a T-handle, or a screwdriver, put it in the open T-slot and lift up, and it’ll pop right out for you. Now one other style we want to talk about is our aluminum style. It installs the same way, except you may want to use a soft-faced mallet to pop it into place. One other thing with this T-slot cover, it it’s a little more rigid, so once you have it installed you want to be careful taking it out because you can bend it easily. Now hopefully you found this segment on T-slot cover helpful. For more information, please visit 8020.net and be sure to subscribe to future 80/20 videos. And as always, make it a great day!

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#dotblot #RohitMane #ScientistRacademy Dot Blot is a cheaper, easier and faster technique to detect the presence of Proteins and Nucleic Acids in a biological sample. Due to the simplicity of the technique it widely used as a ideal diagnostic tool. This post covers the Protein Dot Blot Technique. Dot Blot Principle Dot Blot works based on the immunodetection principle for identifying specific protein. Dot Blot - PrincipleDot Blot - Technique: Overview Proteins are immobilized on to a protein binding membrane, PVDF or Nitrocellulose. After Protein Immobilization membrane is blocked to avoid non-specific binding, using blocking buffer. Immobilized protein is then incubated with a primary antibody (for 30mins to 1 hour). The antibody is specific to the protein and is visualized either with a specific tag or with a secondary antibody.  The secondary antibody generally will carry a tag that allow the visualization of protein. Blots can be visualized using methods such as Chemiluminescence or Fluorescence. Common Tags used in Dot Blots are enzymes conjugated to the secondary antibody. Upon addition of substrate, enzyme acts on the substrate to develop colour. Commonly preferred enzymes are HRP(Horse Radish Peroxidase) and Alkaline Phosphatase (AP) Advantages of Dot Blot over Western Blot Non-Electrophoresis Technique: Dot Blot is non-electrophoresis technique, means that one need not do protein electrophoresis. Proteins can directly immobilized on to the membrane. Simple & Easy Method: No messy work of PAGE run and gel to membrane transfers, proteins are directly immobilized on membrane, making this method much easier and faster. Time: It is a faster method, takes ~3hours, whereas western blot may even take up to 2 days. Including the protein electrophoresis and gel to membrane transfer optimization. Dot Blot is not as informative as Western Blot, but it is inexpesive, quick and easy method for the simple protein detection.  dot blot technique by shomus biology dot blot technique in hindi

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